中国水稻科学

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (1): 9-14.DOI: 10.3969/j.issn.1001-7216.2014.01.002

• 研究报告 • 上一篇    下一篇

稻曲病菌UvHog1基因的克隆及表达分析

饶玉春1,2,# ,丁正中1,3,# ,陈析丰1 ,曾大力2 ,马伯军1,顾志敏 1,*   

  1. 1浙江师范大学 化学与生命科学学院， 浙江 金华 321004； 2中国水稻研究所 水稻生物学国家重点实验室， 杭州 310006； 3金华市
    艾青中学， 浙江 金华 321015；
  • 收稿日期:2013-09-16 修回日期:2013-11-15 出版日期:2014-01-10 发布日期:2014-01-10
  • 通讯作者: 顾志敏1,*
  • 基金资助:

    国家自然科学基金资助项目（31240079； 30800677; 31201183）；浙江省自然科学基金资助项目（LY12C06001）；浙江省重中之重学科开放基金资助项目。

Cloning and Expression Analysis of UvHog1 Gene in  Ustilaginoidea virens

RAO Yuchun 1,2,# , DING Zhengzhong 1,3,# , CHEN Xifeng1 , ZENG Dali 2 , MA Bojun1 , GU Zhimin 1,*   

  1. 1College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua  321004,  China;  2 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; 3 Jinhua Aiqing School, Jinhua 321015, China;
  • Received:2013-09-16 Revised:2013-11-15 Online:2014-01-10 Published:2014-01-10
  • Contact: GU Zhimin1,*

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摘要： 稻曲病由稻曲病菌引起，是水稻穗部的重要病害。利用同源克隆和RACE技术，根据丝状真菌相对保守的氨基酸序列设计简并引物，从稻曲病菌中分离了丝裂原活化蛋白激酶（MAPK）编码基因的全长cDNA序列，命名为UvHog1。 UvHog1含有MAPK保守的蛋白激酶激活域（TGY），序列与稻瘟病菌MgOsm1 （AAF09475.1）、球孢白僵菌BbHog1（AAS77871.1）、烟曲霉AfOsm1 （XP752664.1） 和隐球酵母CrHog1（AAM26267.1）等MAPK高度同源，相似性分别为95%、93%、88%和81%。系统聚类结果表明，UvHog1与酵母Hog1 MAPK同源。在盐胁迫条件下，UvHog1的相对表达量明显降低，表明UvHog1参与了对盐胁迫的信号响应。

关键词: 稻曲病菌, Hog1, 克隆, 表达分析

Abstract: Rice false smut, caused by Ustilaginoidea virens,  is an important disease in rice panicle. Degenerate PCR primers were designed according to the conserved amino acid sequence of several filamentous fungus mitogen activated protein kinase(MAPK) homologous to yeast Hog1 MAPK, and partial DNA fragment encoding a MAPK was amplified from U. virens through PCR. Then, a whole DNA sequence encoding the MAPK, designated as UvHog1, was obtained from U. virens by extending upstream and downstream sequence of the amplified fragment using RACE method. And its amino acid sequence shared 95%, 93%, 88% and 81% identity to MgOsm1 （AAF09475.1） from Magnaporthe grisea,  BbHog1 （AAS77871.1） from Beauveria bassiana,  AfOsm1（XP752664.1）from Aspergillus fumigatus and CrHog1（AAM26267.1）from Cryptococcus neoformans var. neoformans, respectively. Phylogenetic clustering suggested that UvHog1 is homologous to yeast Hog1 MAPK. The results of the decreased transcript levels of UvHog1 by salinity suggest that UvHog1 may be involved in the specifically responses to salt stresses.

Key words: Ustilaginoidea virens, Hog1, cloning, expression analysis

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